pnpp one component microwell substrate solution (SouthernBiotech)

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SouthernBiotech pnpp one component microwell substrate solution
Pnpp One Component Microwell Substrate Solution, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pnpp+substrate+solution/pNPP+One+Component+Microwell+Substrate/pm42873867-343-9-15
Average 93 stars, based on 21 article reviews

pnpp one component microwell substrate solution - by Bioz Stars, 2026-11

93/100 stars

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Incubation:

Article Title: A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum.
Article Snippet: .. Several concentrations of rRpPGRP were added in the presence of 5mM CaCl2 and 0.1mg/mL BSA and incubated at 18 °C for 3 h. Following a further washing, 100 μL of 1:1000 diluted polyclonal antibody against rRpPGRP was added into the plate and incubated at 37 °C for 1 h. After three washes, these wells were then incubated in a goat-anti-mouse IgG-alkaline phosphatase conjugated with secondary antibody (Southern Biotech, USA) for 1 h. After the last washing, pNPP substrate solution was added and incubated at room temperature in dark. ..

Article Title: Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions.
Article Snippet: .. The plates were washed for three times and incubated with diluted polyclonal antibody against rRpSR-BI (1:1000 diluted in PBS) at 37 °C for 1 h. Afterwards, the plates were washed again and 100 μL of goat-antimouse Ig-alkaline phosphatase conjugate (1:3000 diluted in PBS, Southern Biotech, USA) was added and incubated at 37 °C for 1 h. After the last washing, pNPP substrate solution was added and incubated at room temperature in dark. ..

Article Title: ACVR1-Fc suppresses BMP signaling and chondro-osseous differentiation in an in vitro model of Fibrodysplasia ossificans progressiva.
Article Snippet: .. After another 2-hour incubation at 37 °C, the plates were washed again and added 50 μl of PNPP substrate solution (Southern Biotech). .. Finally the plates were read at double wavelength 405 nm and 490 nm using a microplate reader (iMax, Bio-Rad).

Article Title: Recombinant Salmonella Expressing Burkholderia mallei LPS O Antigen Provides Protection in a Murine Model of Melioidosis and Glanders
Article Snippet: .. Secondary antibodies (anti-mouse total IgG, IgG1, IgG2a, IgG2b, IgG3, or IgM conjugated to alkaline phosphatase (Southern Biotechnology Associates, Inc., Birmingham, AL)) were added to individual plates, diluted 1:5000 in PBS-B, and incubated at 37°C for 1 h. The microwells were washed again, and the enzymatic reaction was developed by the addition of 4-nitrophenol phosphate disodium salt hexahydrate (PNPP) diluted to 1 mg/ml in PNPP substrate solution (10% diethanolamine, 25 μM MgCl 2 ). .. The reaction was terminated by the addition of 3 M NaOH to each well, and the results were recorded by using a Thermo microplate reader (Molecular Devices, CA) measuring the colorimetric reaction at OD 405 with the data was displayed using SOFTmax Pro version 1.1 software.

Enzyme-linked Immunosorbent Assay:

Article Title: Intrinsic T cell glutaminolysis promotes autoimmunity in lupus-prone mice
Article Snippet: .. IgG was measured by ELISA using plates coated with goat anti-mouse IgG (Accurate Chemical) at a concentration of 1 μg/mL in 0.1 M bicarbonate buffer and detected with alkaline phosphatase-conjugated goat anti-mouse IgG (Millipore/Sigma) diluted 1:1,000 and of PNPP substrate solution (Southern Biotech). ..

Concentration Assay:

other:

Article Title: Co-adjuvanting Nod2-stimulating bacteria with a TLR7 agonist elicits potent protective immunity against respiratory virus infection.
Article Snippet: Objectives: This study investigates the synergistic effect of combining the TLR7 agonist Imiquimod with either the Nod2 agonist (muramyl dipeptide; MDP) or commensal bacteria as nasal vaccine adjuvants to enhance immunity against respiratory viruses.. Methods: Mice assessed immune responses, including antibody and cytokine profiles, after intranasal immunization with antigen and adjuvant combinations.. BMDCs were cultured with these components to measure cytokine production.

Review